Conjugation

Part:BBa_K1682014

Designed by: Zilu Peter Tang   Group: iGEM15_HKUST-Rice   (2015-09-08)


Conjugation(RP4) plasmid with partial GFP

This plasmid contains three main components: the RP4 and GFP. The RP4 gene, originally from pRZ101, when transformed specifically into S17 E. coli strain, facilitates the conjugation of E.coli with most other bacteria. It consists of TraJR BBa_J01001 and oriT BBa_K125320. The GFP is from biobrick BBa_E0040. The plasmid also contains biobrick part BB0030 RBS, and BBa_0014 Terminator.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 638
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1015
    Illegal NgoMIV site found at 1141
    Illegal NgoMIV site found at 1151
    Illegal AgeI site found at 450
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 672
    Illegal SapI site found at 1043


[edit]
Categories
Parameters
None